Journal: Microbial Biotechnology

Article Title: Aloe‐emodin‐mediated antimicrobial photodynamic therapy against dermatophytosis caused by Trichophyton rubrum

doi: 10.1111/1751-7915.13875

Figure Lengend Snippet: Photodynamic treatment effects of AE for tinea corporis in vivo and tinea unguium ex vivo . A. Photographs of infected skin on the dorsum of guinea pigs; (P−L−): treated with sterile PBS; (P−L+): irradiated with 48 J cm −2 of 435 ± 10 nm light; (P + L−): treated with 10 μM AE in the dark for 2 h; (P + L+): treated with 10 μM AE in the dark for 2 h and irradiated with 48 J cm −2 light. B. Lesion score of infected skin on the dorsum of guinea pigs. C. The amounts of T. rubrum cells on the surface of infected skin after 1 time treatment. D. The amounts of T. rubrum cells on the surface of infected skin after 3 times of treatments. E. The amount of T. rubrum cells in the infected skin tissue after 3 times of treatments. F. CFW staining images of un‐infected skin (NC), infected skin treated with sterile PBS (P−L−) and infected skin treated with 10 μM AE and irradiated with 48 J cm −2 light for 3 times (P + L+). G. Effectiveness of AE‐mediated aPDT in onychomycosis ex vivo model. (P−L−): treated with sterile PBS; (P−L+): irradiated with 48 J cm −2 of 435 ± 10 nm light; (P + L−): treated with 10 μM AE in the dark for 2 h; (P + L+): treated with 10 μM AE in the dark for 2 h and irradiated with 48 J cm −2 light. Each value refers to mean ± standard deviation (SD) ( n = 3) (ns: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: Recently, they have developed a guinea pig onychomycosis in vivo model using the similar method (Hasegawa and Shibuya, ).

Techniques: In Vivo, Ex Vivo, Infection, Sterility, Irradiation, Staining, Standard Deviation

Journal: Cell reports

Article Title: Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse

doi: 10.1016/j.celrep.2019.02.070

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: table ft1 table-wrap @position="anchor" mode=article t4 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Polyclonal guinea pig γ2-GABA A R Synaptic systems Cat# 224004; RRID: AB_10594245 Monoclonal mouse anti-gephyrin Synaptic systems Cat# 147011; RRID: AB_887717 Polyclonal rabbit VGAT Synaptic systems Cat# 131003; RRID: AB_887869 Polyclonal rabbit RIM1/2 Synaptic systems Cat# 140203; RRID: AB_887775 STAR 580 Goat anti-guinea pig Abberior Cat#: 2–0112-005–7 STAR 635 Goat anti-mouse Abberior Cat#: 2–0002-002–0 Goat anti-guinea pig Alexa Fluor-568 Life Technologies Cat# A11075; RRID: 141954 Donkey anti-mouse AlexaFluor-488 Life Technologies Cat# A21202; RRID: 141607 Donkey anti-rabbit Alexa Fluor-647 Life Technologies Cat# {"type":"entrez-protein","attrs":{"text":"A31573","term_id":"87384","term_text":"pir||A31573"}} A31573 ; RRID: 2536183 Chemicals, Peptides, and Recombinant Proteins (-)-Bicuculline methiodide Tocris Cat# 2503 Experimental Models: Organisms/Strains Rat, Sprague Dawley Charles River Charles Rivers Crl:CD(SD)BR; RRID: RGD_734476 C57BL/6J mice Jackson Laboratories IMSR_JAX:000664 Recombinant DNA Gephyrin FingR Gross et al., 2013 Addgene # 46296 Software and Algorithms ImageJ National Institutes of Health https://imagej.nih.gov/ij/ NIS Elements Nikon https://www.nikoninstruments.com/Products/Software Prism GraphPad https://www.graphpad.com/scientific-software/prism/ Mosaic Suite (FIJI/ImageJ plugin) Mosaic Group http://mosaic.mpi-cbg.de/?q=downloads/imageJ MATLAB Mathworks http://www.mathworks.com Open in a separate window KEY RESOURCES TABLE CONTACT FOR REAGENT AND RESOURCE SHARING Information about and requests for reagents and resources should be directed to and will be fulfilled by the Lead Contact, Katharine Smith ( ude.revnedcu@htims.r.enirahtak ).

Techniques: Recombinant, Software